4BVX

Crystal structure of the AIMP3-MRS N-terminal domain complex with I3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-10
Detector	ADSC CCD
Spacegroup name	P 2 21 21
Unit cell lengths	43.284, 71.584, 116.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.490 - 1.600
R-factor	0.21833
Rwork	0.217
R-free	0.24657
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.308
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.100	0.540
Number of reflections	43517
<I/σ(I)>	24.4	3.62
Completeness [%]	89.2	94.4
Redundancy	9.6	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.7		PROTEIN WAS CRYSTALLIZED FROM 18% PEG 3350, 10 MM DTT, 100 MM HEPES, PH 7.7; THEN SOAKED IN 200 MM I3C.