4BVU
Structure of Shigella effector OspG in complex with host UbcH5c- Ubiquitin conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 183.873, 183.873, 63.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.160 - 2.700 |
| R-factor | 0.24658 |
| Rwork | 0.244 |
| R-free | 0.29062 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x23 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.200 | 1.000 |
| Number of reflections | 17947 | |
| <I/σ(I)> | 16.39 | 2.58 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 19.1 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS PH 8.5, 20% ETOH |
