4BVA
Crystal structure of the NADPH-T3 form of mouse Mu-crystallin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.240, 97.140, 75.670 |
| Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
| Resolution | 42.680 - 1.750 |
| R-factor | 0.14857 |
| Rwork | 0.146 |
| R-free | 0.19224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i99 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 63604 | |
| <I/σ(I)> | 14.28 | 3.69 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 3.76 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM KH2PO4, 15% PEG 3350, 280 MM NASCN |
