4BV9
Crystal structure of the NADPH form of mouse Mu-crystallin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.840, 86.590, 93.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.984 - 2.193 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i99 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.852 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.980 | 2.290 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 30724 | |
| <I/σ(I)> | 9.96 | 3.85 |
| Completeness [%] | 95.4 | 91.1 |
| Redundancy | 2.8 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100 MM BIS TRIS PH 5.5, 23% PEG 3350, 100 MM AMMONIUM SULFATE |
