4BV8
Crystal structure of the apo form of mouse Mu-crystallin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.280, 96.550, 76.080 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 48.280 - 2.300 |
| R-factor | 0.19495 |
| Rwork | 0.192 |
| R-free | 0.24327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i99 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 28087 | |
| <I/σ(I)> | 13.4 | 3.52 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 MM TRIS-CL PH 8.5, 180 MM KH2PO4, 14.4% PEG 3350, 240 MM NASCN |
