4BUP
A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-05-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.310, 50.010, 129.400 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 46.640 - 2.166 |
| R-factor | 0.2012 |
| Rwork | 0.198 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4au7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.170 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 28977 | |
| <I/σ(I)> | 13 | 4.1 |
| Completeness [%] | 90.9 | 90.2 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES, 10 % PEG 10K, pH 7.5 |
