4BTS
THE CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH EIF1 AND EIF1A
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 209.990, 471.550, 298.540 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 49.749 - 3.703 |
| R-factor | 0.2021 |
| Rwork | 0.202 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XZM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.186 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.930 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.150 | 0.770 |
| Number of reflections | 607573 | |
| <I/σ(I)> | 7.69 | 1.66 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 3.1 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 0.1 M MOPS-KOH PH 7.7, 0.1 M AMMONIUM SULPHATE, 7.5% PEG 8K, 1MM PUTRESCINE |
