4BQM
Crystal structure of human liver-type glutaminase, catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 203.613, 203.613, 98.965 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.920 - 2.180 |
| R-factor | 0.17833 |
| Rwork | 0.177 |
| R-free | 0.20324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ss4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.435 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.830 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.070 | 0.530 |
| Number of reflections | 40642 | |
| <I/σ(I)> | 23.5 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M TRIS PH 8.5, 0.2M SODIUM CHLORIDE, 25%(W/V) PEG 3350 |
