4BQA
Crystal structure of the ETS domain of human ETS2 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.674, 96.964, 83.803 |
| Unit cell angles | 90.00, 97.06, 90.00 |
Refinement procedure
| Resolution | 48.482 - 2.500 |
| R-factor | 0.2349 |
| Rwork | 0.234 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zp5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 20136 | |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS TRIS PH 5.5, 0.25 M NACL, 15% PEG 3350 |
