4BPU
Crystal structure of human primase in heterodimeric form, comprising PriS and truncated PriL lacking the C-terminal Fe-S domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-25 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 114.550, 68.640, 126.760 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 29.617 - 2.700 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.24540 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 0.954 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.050 | 0.600 |
Number of reflections | 52124 | |
<I/σ(I)> | 10.2 | 1.6 |
Completeness [%] | 98.6 | 99.8 |
Redundancy | 2.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100MM TRIS-HCL/BICINE PH 8.5, 20% GLYCEROL, 10% PEG4000 AND 20MM EACH OF AN ALCOHOL MIX COMPRISING 1,6-HEXANEDIOL, 1-BUTANOL, 1,2-PROPANEDIOL, 2-PROPANOL, 1,4- BUTANEDIOL AND 1,3-PROPANEDIOL |