4BPU
Crystal structure of human primase in heterodimeric form, comprising PriS and truncated PriL lacking the C-terminal Fe-S domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-25 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.550, 68.640, 126.760 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 29.617 - 2.700 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24540 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.954 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.960 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 52124 | |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 98.6 | 99.8 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS-HCL/BICINE PH 8.5, 20% GLYCEROL, 10% PEG4000 AND 20MM EACH OF AN ALCOHOL MIX COMPRISING 1,6-HEXANEDIOL, 1-BUTANOL, 1,2-PROPANEDIOL, 2-PROPANOL, 1,4- BUTANEDIOL AND 1,3-PROPANEDIOL |
