4BPT
Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.320, 60.120, 124.040 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.26481 |
| Rwork | 0.264 |
| R-free | 0.30203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v27 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.083 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 41892 | |
| <I/σ(I)> | 0 | 2.2 |
| Completeness [%] | 90.4 | 94 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M NAK2PO4, 4% (V/V) PEG 400 |
