4BOZ
Structure of OTUD2 OTU domain in complex with K11-linked di ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 287 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.790, 44.220, 84.950 |
| Unit cell angles | 90.00, 91.41, 90.00 |
Refinement procedure
| Resolution | 43.684 - 3.030 |
| R-factor | 0.1964 |
| Rwork | 0.193 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4boq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.156 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.190 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 12691 | |
| <I/σ(I)> | 7.1 | 3.3 |
| Completeness [%] | 97.9 | 98.8 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M POTASSIUM THIOCYANATE, 18% PEG 5K MME, 0.1 M SODIUM ACETATE PH 5.5 |
