Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 64.883, 64.883, 129.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.020 - 2.900 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22270 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.800 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 1.270 |
| Number of reflections | 6594 | |
| <I/σ(I)> | 17.6 | 1.9 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 8.9 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SEMET PROTEIN/DNA WAS CRYSTALLISED IN 20%(W/V) PEG 3350 0.2M POTASSIUM CITRATE. NATIVE PROTEIN/DNA WAS CRYSTALLISED IN 28% LMW PEG SMEAR, 100MM TRIS, PH 8.5, 200MM NACL, 5% GLYCEROL |
