4BM1
CRYSTAL STRUCTURE OF MANGANESE PEROXIDASE 4 FROM PLEUROTUS OSTREATUS - CRYSTAL FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.990, 75.370, 75.610 |
| Unit cell angles | 69.75, 75.69, 75.82 |
Refinement procedure
| Resolution | 38.148 - 1.098 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.14730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmu |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.710 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 271193 | |
| <I/σ(I)> | 13.5 | 1.9 |
| Completeness [%] | 83.9 | 43 |
| Redundancy | 13.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-CITRATE AT PH 5.5 & 2.0 M (NH4)2SO4. |
