4BLA
Crystal structure of full-length human Suppressor of fused (SUFU) mutant lacking a regulatory subdomain (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 117.920, 372.570, 86.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.577 - 3.500 |
R-factor | 0.2608 |
Rwork | 0.259 |
R-free | 0.29320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4BL8 1omp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.249 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.580 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.240 | |
Number of reflections | 49033 | |
<I/σ(I)> | 28.1 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.7 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PROTEIN (11.6 MG/ML IN 10 MM TRIS-HCL PH 7.5, 50 MM NACL, 1 MM DTT) WAS CRYSTALLISED BY HANGING DROP VAPOUR DIFFUSION AT 4C WITH 0.1 M NA-HEPES PH 7.5, 17% (V/V) PEG 3350, 0.2 M NACL. |