4BL0
Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.740, 57.900, 118.670 |
Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
Resolution | 46.882 - 1.950 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 213S |
RMSD bond length | 0.008 |
RMSD bond angle | 0.964 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 1.050 |
Number of reflections | 66488 | |
<I/σ(I)> | 14.15 | 3 |
Completeness [%] | 98.7 | 88.1 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.2M K/ NA TARTRATE, 0.1M BIS TRIS PROPANE PH 7.5, 20% PEG 3350 |