4BKP
Crystal structure of human GDP-L-fucose synthase with bound NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 106.596, 163.095, 197.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.440 - 2.700 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23091 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 1.070 |
| Number of reflections | 47605 | |
| <I/σ(I)> | 14.9 | 1.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.1M CITRATE PH 5.5 |
