4BJ3

Integrin alpha2 I domain E318W-collagen complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-15
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	83.004, 83.004, 175.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.346 - 3.042
R-factor	0.251
Rwork	0.246
R-free	0.29770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dzi
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	3.250
High resolution limit [Å]	3.040	3.040
Rmerge	0.110	0.870
Number of reflections	12365
<I/σ(I)>	15.2	2.9
Completeness [%]	99.2	96.5
Redundancy	13.4	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1