4BDR

Crystal structure of the GluK2 R775A LBD dimer in complex with kainate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 2 2 2
Unit cell lengths	95.503, 105.331, 112.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.766 - 1.650
R-factor	0.1657
Rwork	0.164
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xxt
RMSD bond length	0.006
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.770	1.670
High resolution limit [Å]	1.650	1.650
Rmerge	0.040	0.680
Number of reflections	66575
<I/σ(I)>	21.2	2.2
Completeness [%]	97.7	99.8
Redundancy	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				23% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE
1				23% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE