4BDQ

Crystal structure of the GluK2 R775A LBD dimer in complex with glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	95.753, 105.585, 114.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.644 - 1.900
R-factor	0.1679
Rwork	0.166
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xxr
RMSD bond length	0.007
RMSD bond angle	0.985
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.640	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.820
Number of reflections	45850
<I/σ(I)>	14.6	1.9
Completeness [%]	99.6	99.2
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				21% PEG 4,000, 80MM NA ACETATE
1				21% PEG 4,000, 80MM NA ACETATE