4BDO
Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.838, 99.819, 124.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.785 - 2.550 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.790 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.700 |
| Number of reflections | 35522 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 27% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE |
