4BDN

Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	85.729, 100.133, 126.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.561 - 2.500
R-factor	0.2001
Rwork	0.198
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xxr
RMSD bond length	0.006
RMSD bond angle	0.721
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.560	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.620
Number of reflections	38135
<I/σ(I)>	12.4	2.6
Completeness [%]	99.6	99.8
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE
1				25% PEG 4,000, 6% PROPAN-2-OL, 80MM NA ACETATE