4BD4
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, mutant H43F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-18 |
Detector | MARRESEARCH 165MM |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 235.030, 49.890, 97.940 |
Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
Resolution | 29.148 - 2.780 |
R-factor | 0.1837 |
Rwork | 0.180 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xjk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.284 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.050 | 0.860 |
Number of reflections | 28537 | |
<I/σ(I)> | 16.3 | 2.1 |
Completeness [%] | 98.2 | 99.3 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 18 % PEG 1500, 15% GLYCEROL |