4BD4
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, mutant H43F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARRESEARCH 165MM |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.030, 49.890, 97.940 |
| Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
| Resolution | 29.148 - 2.780 |
| R-factor | 0.1837 |
| Rwork | 0.180 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.284 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.050 | 0.860 |
| Number of reflections | 28537 | |
| <I/σ(I)> | 16.3 | 2.1 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % PEG 1500, 15% GLYCEROL |
