4BBQ
Crystal structure of the CXXC and PHD domain of Human Lysine-specific Demethylase 2A (KDM2A)(FBXL11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.024, 30.053, 90.824 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 90.790 - 2.240 |
| R-factor | 0.1981 |
| Rwork | 0.197 |
| R-free | 0.22090 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | autoPROC |
| Data scaling software | AP_SCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.790 | 2.360 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 13091 | |
| <I/σ(I)> | 16 | 3.8 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M BIS-TRIS, PH 6.5, 20%(W/V) PEG METHYL ETHER 5000 |
