4BBD
THE STRUCTURE OF VACCINIA VIRUS N1 R58Y MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | MARRESAERCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.560, 110.200, 72.930 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 42.790 - 3.000 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i39 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BUSTER-TNT |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | |
| Number of reflections | 20904 | |
| <I/σ(I)> | 20 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
