2I39

Crystal structure of Vaccinia virus N1L protein

Summary for 2I39

• Entry DOI: 10.2210/pdb2i39/pdb
• Descriptor: Protein N1, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total)
• Functional Keywords: all alpha, viral protein
• Biological source: Vaccinia virus
• Total number of polymer chains: 6
• Total formula weight: 97132.80

Authors

Aoyagi, M.,Aleshin, A.E.,Stec, B.,Liddington, R.C. (deposition date: 2006-08-17, release date: 2006-11-21, Last modification date: 2024-02-21)

Primary citation

Aoyagi, M.,Zhai, D.,Jin, C.,Aleshin, A.E.,Stec, B.,Reed, J.C.,Liddington, R.C.
Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family protein.
Protein Sci., 16:118-124, 2007

PubMed Abstract: Poxviruses encode immuno-modulatory proteins capable of subverting host defenses. The poxvirus vaccinia expresses a small 14-kDa protein, N1L, that is critical for virulence. We report the crystal structure of N1L, which reveals an unexpected but striking resemblance to host apoptotic regulators of the B cell lymphoma-2 (Bcl-2) family. Although N1L lacks detectable Bcl-2 homology (BH) motifs at the sequence level, we show that N1L binds with high affinity to the BH3 peptides of pro-apoptotic Bcl-2 family proteins in vitro, consistent with a role for N1L in modulating host antiviral defenses.

PubMed: 17123957
DOI: 10.1110/ps.062454707

Experimental method

X-RAY DIFFRACTION (2.2 Å)