4BA1
Archaeal exosome (Rrp4-Rrp41(D182A)-Rrp42) bound to inorganic phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-29 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 3 |
Unit cell lengths | 135.070, 135.070, 135.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.750 - 1.800 |
R-factor | 0.18576 |
Rwork | 0.185 |
R-free | 0.22171 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2je6 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.691 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.650 |
Number of reflections | 71709 | |
<I/σ(I)> | 11.4 | 1.6 |
Completeness [%] | 1.0 | 0.9 |
Redundancy | 5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 40% PEG 400, 50MM TRIS HCL PH 8.0 |