4B9N
Structure of the high fidelity DNA polymerase I correctly bypassing the oxidative formamidopyrimidine-dA DNA lesion.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.452, 93.726, 105.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.892 - 2.200 |
R-factor | 0.1849 |
Rwork | 0.183 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b9m |
RMSD bond length | 0.006 |
RMSD bond angle | 0.993 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.890 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.590 |
Number of reflections | 45055 | |
<I/σ(I)> | 16 | 3.2 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |