4B90
Crystal structure of WT human CRMP-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.150, 90.150, 247.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.370 - 2.200 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.22082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b91 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.738 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.600 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.720 |
| Number of reflections | 69984 | |
| <I/σ(I)> | 10.4 | 10.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25% (W/V) PEG 1500 SPG PH 6.0 |
