4B7W

Ligand binding domain human hepatocyte nuclear factor 4alpha: Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-06
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	101.048, 105.282, 98.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.000
R-factor	0.2496
Rwork	0.250
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lv2
RMSD bond length	0.002
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	4.240
High resolution limit [Å]	4.000	4.000
Rmerge	0.160	0.810
Number of reflections	8011
<I/σ(I)>	7.1	1.8
Completeness [%]	88.5	89
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.7 M AMMONIUM ACETATE, 0.1 M SODIUM CITRATE, 10 MM DTT, 16% MPD IN 0.1 M TRIS, PH 8.0