Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 155.290, 154.779, 58.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.960 - 1.880 |
| R-factor | 0.17689 |
| Rwork | 0.176 |
| R-free | 0.20059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x8l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.090 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.010 | 0.880 |
| Number of reflections | 115329 | |
| <I/σ(I)> | 16.8 | 1.9 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.4 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M BICINE PH 9, 22.5% PEG6000 |
