4B7E

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH CONSENSUS ANKYRIN REPEAT DOMAIN-LEU PEPTIDE (20-MER)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-23
Detector	RIGAKU SATURN 944
Spacegroup name	P 41 21 2
Unit cell lengths	86.448, 86.448, 143.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.120 - 2.500
R-factor	0.226
Rwork	0.226
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.120	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.850
Number of reflections	19147
<I/σ(I)>	16.7	2.7
Completeness [%]	97.6	97
Redundancy	6.4	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH 7.5