4B5M

Neisseria AP endonuclease bound to the substrate with a cytosine orphan base

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Spacegroup name	P 41 21 2
Unit cell lengths	77.050, 77.050, 382.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.102 - 2.758
R-factor	0.2325
Rwork	0.230
R-free	0.27670
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.005
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.100
High resolution limit [Å]	2.760
Rmerge	0.060
Number of reflections	30548
Completeness [%]	98.5
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1