4B2Z
Structure of Osh6 in complex with phosphatidylserine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-16 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.000, 72.600, 122.900 |
Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
Resolution | 46.566 - 1.950 |
R-factor | 0.1982 |
Rwork | 0.198 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zht |
RMSD bond length | 0.008 |
RMSD bond angle | 1.219 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.060 | 0.650 |
Number of reflections | 65896 | |
<I/σ(I)> | 18 | 1.6 |
Completeness [%] | 91.8 | 59 |
Redundancy | 3.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1M MES PH6.5, 13% PEG6000, 5% MPD |