4B21
Unprecedented sculpting of DNA at abasic sites by DNA glycosylase homolog Mag2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 68.926, 84.746, 126.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.450 |
R-factor | 0.2069 |
Rwork | 0.207 |
R-free | 0.22080 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.005 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 62545 | |
<I/σ(I)> | 15.2 | 4.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.40 M (NH4)H2PO4 (NOT PH ADJUSTED), 6% 1,6-HEXANEDIOL |