4B1G
Structure of unliganded human PARG catalytic domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.979, 0.975, 0.982 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.759, 66.387, 89.323 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 66.430 - 1.830 |
| R-factor | 0.18492 |
| Rwork | 0.183 |
| R-free | 0.21794 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.444 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.990 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 39497 | |
| <I/σ(I)> | 12.4 | 2 |
| Completeness [%] | 86.3 | 47.9 |
| Redundancy | 6.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 28% PEG3350, 0.2M MAGNESIUM CHLORIDE, 0.1M PCTP (0.04M SODIUM PROPIONATE, 0.02M SODIUM CACODYLATE, 0.04M BIS-TRIS PROPANE) PH 7.5; 1:1 RATIO; 4 MICROLITRE DROP; ROOM TEMPERATURE |
