4AYH
The X-ray structure of zinc bound ZinT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.398, 58.398, 291.461 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.570 - 2.520 |
| R-factor | 0.23789 |
| Rwork | 0.234 |
| R-free | 0.32208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 10EK |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.611 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.180 | 0.470 |
| Number of reflections | 10654 | |
| <I/σ(I)> | 15.02 | 3.83 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 5.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | AMMONIUM SULPHATE 1.5 M, SODIUM ACETATE 0.1 M PH 4.6 |
