4AYC
RNF8 RING domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2010-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.374, 213.717, 34.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.290 - 1.900 |
| R-factor | 0.20136 |
| Rwork | 0.200 |
| R-free | 0.22948 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.370 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.600 |
| Number of reflections | 28115 | |
| <I/σ(I)> | 25.8 | 2.7 |
| Completeness [%] | 99.1 | 95.2 |
| Redundancy | 6.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 0.1 M NA CACODYLATE, PH 6.9, 2.5 M AMMONIUM SULPHATE, 0.8 MM N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE |
