4AYB
RNAP at 3.2Ang
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-11-06 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 195.725, 212.415, 128.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.097 - 3.202 |
R-factor | 0.2454 |
Rwork | 0.242 |
R-free | 0.30050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2waq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.762 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.100 | 3.270 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.180 | |
Number of reflections | 88449 | |
<I/σ(I)> | 13.9 | 2 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 15.2 | 15.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |