4AXV
Biochemical and structural characterization of the MpaA amidase as part of a conserved scavenging pathway for peptidoglycan derived peptides in gamma-proteobacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-10 |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.600, 73.600, 208.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.960 - 2.170 |
R-factor | 0.19761 |
Rwork | 0.196 |
R-free | 0.23293 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.020 |
RMSD bond angle | 2.038 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELX (CDE) |
Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.570 | 2.230 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.100 | 0.780 |
Number of reflections | 18606 | |
<I/σ(I)> | 33.5 | 7.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 40.8 | 42.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8 |