4AXK
CRYSTAL STRUCTURE OF subHisA from the thermophile Corynebacterium efficiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.460, 78.840, 92.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.640 - 2.250 |
| R-factor | 0.23813 |
| Rwork | 0.236 |
| R-free | 0.30263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vep |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 1.120 |
| Number of reflections | 24561 | |
| <I/σ(I)> | 16.2 | 2 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M BIS-TRIS PH 7.5, 25% V/W PEG-3350, 0.2 M MGCL2,30% GLYCEROL |
