4AX4
PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, H680A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.080, 99.080, 110.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.100 - 1.600 |
| R-factor | 0.16368 |
| Rwork | 0.162 |
| R-free | 0.19577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qfm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.780 |
| Number of reflections | 101085 | |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18-20% METHOXY-POLYETHYLENE GLYCOL (MPEG) 5K, 20 MM CA(OAC)2, 0.1 M TRIS PH 8.5, 15% GLYCEROL |
