4AWN
Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2008-04-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.960, 58.660, 96.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.125 - 1.950 |
R-factor | 0.169 |
Rwork | 0.166 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dni |
RMSD bond length | 0.013 |
RMSD bond angle | 1.428 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.100 | 0.570 |
Number of reflections | 18022 | |
<I/σ(I)> | 6.88 | 1.32 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.48 | 7.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP VAPOR DIFFUSION, 0.2 M MGCL2, 0.1 M BIS-TRIS AT PH 5.5, 25% V/V PEG 3350 |