4AW6
Crystal structure of the human nuclear membrane zinc metalloprotease ZMPSTE24 (FACE1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.987, 95.451, 131.095 |
| Unit cell angles | 76.73, 79.64, 72.61 |
Refinement procedure
| Resolution | 37.960 - 3.400 |
| R-factor | 0.2466 |
| Rwork | 0.246 |
| R-free | 0.26420 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | BUSTER-TNT (BUSTER 2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.450 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.130 | 1.360 |
| Number of reflections | 37469 | |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES, PH 7.5, 0.1 M CALCIUM CHLORIDE, 41.5%(V/V) PEG 400 |
