4AVP
Crystal structure of the DNA-binding domain of human ETV1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-29 |
| Detector | MARRESEARCH MARMOSAIC 300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.317, 45.607, 55.406 |
| Unit cell angles | 77.89, 84.80, 90.02 |
Refinement procedure
| Resolution | 53.940 - 1.820 |
| R-factor | 0.2227 |
| Rwork | 0.221 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gvj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | autoPROC |
| Data scaling software | AP_SCALE |
| Phasing software | PHASER (MR) |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.940 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.054 | 0.330 |
| Rpim | 0.062 | 0.360 |
| Number of reflections | 27819 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 97.4 | 96.2 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.5M SODIUM FORMATE, pH 7.5 |
