4AUQ

Structure of BIRC7-UbcH5b-Ub complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Spacegroup name	P 43 21 2
Unit cell lengths	100.640, 100.640, 123.899
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.499 - 2.176
R-factor	0.2044
Rwork	0.202
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3A33 AND 3EB6
RMSD bond length	0.009
RMSD bond angle	1.239
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.180	2.180
Rmerge	0.060	0.620
Number of reflections	33958
<I/σ(I)>	16.5	3.3
Completeness [%]	99.8	99.2
Redundancy	6.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1