4AU9
Crystal Structure of a Fungal DyP-Type Peroxidase from Auricularia auricula-judae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.599, 46.689, 141.198 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.100 |
| R-factor | 0.18364 |
| Rwork | 0.180 |
| R-free | 0.26088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d3q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 49651 | |
| <I/σ(I)> | 11.02 | 3.35 |
| Completeness [%] | 96.9 | 93.4 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.17 M SODIUM ACETATE TRIHYDRATE 0.085 M TRIS HYDROCHLORIDE PH 8.5 25.5% POLYETHYLENE GLYCOL 4000 15% GLYCEROL |
