4AU5
Structure of the NhaA dimer, crystallised at low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-24 |
| Detector | MARRESEARCH MX300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.800, 100.560, 141.612 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 29.644 - 3.696 |
| R-factor | 0.3192 |
| Rwork | 0.318 |
| R-free | 0.34200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZCD -FINAL REFINEMENT DONE AGAINST 4ATV |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.100 | 0.820 |
| Number of reflections | 34273 | |
| <I/σ(I)> | 11.7 | 1.2 |
| Completeness [%] | 98.1 | 90 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.8 | 0.1 M SODIUM CITRATE PH 3.8, 0.1 M LITHIUM SULPHATE, 26% PEG 400 WITH 1% HEPTYL-THIOL-B-D-GLUCOSIDE AS AN ADDITIVE |
