4ATN
Crystal structure of C2498 2'-O-ribose methyltransferase RlmM from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-07 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.090, 92.090, 83.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.769 - 1.950 |
| R-factor | 0.1707 |
| Rwork | 0.169 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL OBTAINED FROM K2PTCL4 DERIVATIVE CRYSTAL DATA |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.770 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.870 |
| Number of reflections | 30022 | |
| <I/σ(I)> | 25 | 3.5 |
| Completeness [%] | 99.8 | 96 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.3 M AMMONIUM TARTRATE DIBASIC PH 7.0, 20% PEG 3350, 3.0% DEXTRAN SULFATE SODIUM SALT |
