4ATB
Crystal structure of the NF90-NF45 dimerisation domain complex with CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.158, 83.967, 159.679 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 66.427 - 3.100 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4at7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.873 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 33184 | |
| <I/σ(I)> | 7.6 | 2.8 |
| Completeness [%] | 96.8 | 98.7 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 12-14 % PEG3350, 200 MM MGCL2, 100 MM MES PH 6.5 AND 5 % GLYCEROL |
